====== WGS-assembler ====== Celera Assembler (CA) is a whole-genome shotgun (WGS) assembler for the reconstruction of genomic DNA sequence from WGS sequencing data. * Install wget http://garr.dl.sourceforge.net/project/wgs-assembler/wgs-assembler/wgs-7.0/wgs-7.0-PacBio-Linux-amd64.tar.bz2 bzip2 -dc wgs-7.0-PacBio-Linux-amd64.tar.bz2 | tar -xf - mv wgs-7.0 /usr/local/bin/ chown -R root:root /usr/local/bin/wgs-7.0 * Patch correctPacBio wget http://www.cbcb.umd.edu/software/PBcR/data/correctPacBio mv /usr/local/bin/wgs-7.0/Linux-amd64/bin/correctPacBio /usr/local/bin/wgs-7.0/Linux-amd64/bin/correctPacBio.OLD mv correctPacBio /usr/local/bin/wgs-7.0/Linux-amd64/bin/ chmod 755 /usr/local/bin/wgs-7.0/Linux-amd64/bin/correctPacBio * Test /usr/local/bin/wgs-7.0/Linux-amd64/bin/pacBioToCA usage: /usr/local/bin/wgs-7.0/Linux-amd64/bin/pacBioToCA [options] -s spec.file -fastq fastqfile -length Minimum length to keep. -partitions Number of partitions for consensus -sge Submit consensus jobs to the grid -sgeCorrection Parameters for the correction step for the grid. This should match the threads specified below, for example by using -pe threaded -l libraryname Name of the library; freeformat text. -t threads Number of threads to use for correction.