====== Install Blast+ ======


  * **DB**
Make directory /db and mount it as separated partition.
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Copy update script from Blast+ bin directory.
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<code>
# cp /usr/share/ncbi-blast-2.6.0+/bin/update_blastdb.pl /db/

# apt-get install liblist-moreutils-perl
</code>
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List database names 
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<code>
# cd /db
# ./update_blastdb.pl --showall
</code>
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Download/update database
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<code>
# cd /db
# ./update_blastdb.pl --decompress --verbose swissprot
</code>
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  * **Blast+**

Download and install
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<code>
# wget ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/ncbi-blast-2.6.0+-x64-linux.tar.gz
# tar xzf ncbi-blast-2.6.0+-x64-linux.tar.gz
# mv ncbi-blast-2.6.0+ /usr/share/
# chown -R root:root /usr/share/ncbi-blast-2.6.0+
# changemod -R 755 /usr/share/ncbi-blast-2.6.0+
</code>
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User script to activate Blast+
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<code bash>
export PATH=/usr/share/ncbi-blast-2.6.0+/bin:$PATH
export BLASTDB=/db
</code>
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Test installation
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<code bash>
$ blastp -db swissprot -query testseq.fasta
</code>
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  * **Schedule DB update**

Copy update script in directory /db.
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<code bash condupdatedb.sh>
#!/bin/bash
        cd /db
        date > "update.log"

	#verify lock db
        echo "verifica lock" >> "update.log"
        DIRS=$(find "/home/" -mindepth 1 -maxdepth 1 -type d)
        for user in $DIRS
        do
                filelock=$user"/dblock"
                if [ -e $filelock ] && [ -f $filelock ]
                then
                        echo $filelock" -> NO update" >> "update.log"
                        exit
                fi
        done
        echo "SI Update" >> "update.log"
        ./update_blastdb.pl --decompress swissprot 16SMicrobial refseq_protein >> "update.log"
        echo "DONE" >> "update.log"
        exit
</code>
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Schedule cron job
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<code>
crontab -e

50 12 * * * /db/condupdatedb.sh > /dev/null 2>&1
</code>
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**NB
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Before blast execution, user must create a file named "dblock" in his home directory to avoid update while blast is running.
At the end of the process the file must be removed.**