====== Bowtie and Bowtie2 ====== \\ * **BOWTIE** # apt-get install bowtie $ bowtie --version bowtie version 1.1.2 64-bit Built on lgw01-11 Thu Nov 26 11:11:48 UTC 2015 Compiler: gcc version 5.2.1 20151123 (Ubuntu 5.2.1-25ubuntu1) Options: -O3 -Wl,--hash-style=both -D_FORTIFY_SOURCE=2 -g -O2 -fstack-protector-strong -Wformat -Werror=format-security -g -O2 -fstack-protector-strong -Wformat -Werror=format-security -Wl,-Bsymbolic-functions -Wl,-z,relro Sizeof {int, long, long long, void*, size_t, off_t}: {4, 8, 8, 8, 8, 8} \\ * **BOWTIE2** Fast and sensitive read alignment \\ * Download wget https://netix.dl.sourceforge.net/project/bowtie-bio/bowtie2/2.2.9/bowtie2-2.2.9-linux-x86_64.zip unzip bowtie2-2.2.9-linux-x86_64.zip * Install mv bowtie2-2.2.9 /usr/local/bin/ ln -s /usr/local/bin/bowtie2-2.2.9/bowtie2 /usr/local/bin/ ln -s /usr/local/bin/bowtie2-2.2.9/bowtie2-build /usr/local/bin/ ln -s /usr/local/bin/bowtie2-2.2.9/bowtie2-inspect /usr/local/bin/ chown -R root:root /usr/local/bin/bowtie2-2.2.9 # apt-get install libtbb-dev * Check $ bowtie2 --version /usr/local/bin/bowtie2-2.2.9/bowtie2-align-s version 2.2.9 64-bit Built on localhost.localdomain Thu Apr 21 18:36:37 EDT 2016 Compiler: gcc version 4.1.2 20080704 (Red Hat 4.1.2-54) Options: -O3 -m64 -msse2 -funroll-loops -g3 -DPOPCNT_CAPABILITY Sizeof {int, long, long long, void*, size_t, off_t}: {4, 8, 8, 8, 8, 8} **NOTE** Tophat2 v2.1.1 test_data error with bowtie2 v2.3.0