BWA

BWA is a program for aligning sequencing reads against a large reference genome (e.g. human genome).
It has two major components, one for read shorter than 150bp and the other for longer reads.

  • Install
# wget https://freefr.dl.sourceforge.net/project/bio-bwa/bwa-0.7.15.tar.bz2
# bzip2 -dc bwa-0.7.15.tar.bz2 | tar -xf  -
# mv bwa-0.7.15 /usr/local/
# cd /usr/local/bwa-0.7.15/
# make
# ln -s /usr/local/bwa-0.7.15/bwa /usr/local/bin/
# chown -R root:root /usr/local/bwa-0.7.15
  • Test
$ bwa

Program: bwa (alignment via Burrows-Wheeler transformation)
Version: 0.7.15-r1140
Contact: Heng Li <lh3@sanger.ac.uk>

Usage:   bwa <command> [options]

Command: index         index sequences in the FASTA format
         mem           BWA-MEM algorithm
         fastmap       identify super-maximal exact matches
         pemerge       merge overlapping paired ends (EXPERIMENTAL)
         aln           gapped/ungapped alignment
         samse         generate alignment (single ended)
         sampe         generate alignment (paired ended)
         bwasw         BWA-SW for long queries

         shm           manage indices in shared memory
         fa2pac        convert FASTA to PAC format
         pac2bwt       generate BWT from PAC
         pac2bwtgen    alternative algorithm for generating BWT
         bwtupdate     update .bwt to the new format
         bwt2sa        generate SA from BWT and Occ

Note: To use BWA, you need to first index the genome with `bwa index'.
      There are three alignment algorithms in BWA: `mem', `bwasw', and
      `aln/samse/sampe'. If you are not sure which to use, try `bwa mem'
      first. Please `man ./bwa.1' for the manual.
 
 
rxr/bwa.txt ยท Last modified: 2017/01/12 21:26 by giancarlo

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Giancarlo Birello (g.birello _@_ ceris.cnr.it) and Anna Perin (a.perin _@_ ceris.cnr.it)
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